TABLE 2.

Membrane fatty acid composition^a of Pediococcus cells^b grown at 37°C in TGY or TSB medium until ST

Acyl chainc	% Total fatty acid on:		Tcd (°C)
	TGY	TSB
SFA
C13:0	NDe	0.05	13.5
C14:0	7.14	8.99	23.0
C15:0	0.56	0.13	34.2
C16:0	18.98	17.50	41.0
C17:0	ND	ND	47.8
C18:0	2.37	1.49	58.0
C20:0	0.33	0.19	66.0
Totalf	29.38 ± 8.70	28.35 ± 7.79
USFA
C16:1 n9c	15.56	18.02	−36.0
C18:2 n9c, n12c	0.39	0.16	−17.6
C18:1 n9c	4.35	2.90	−22.0
C18:1 n11c	40.91	24.97	−19.0
Totalf	61.22 ± 12.45	46.05 ± 7.37
CFA
C17:0 Δ9c	0.26	1.88	−19.9
C19:0 Δ9c	9.15	23.60	−0.5
Totalf	9.41 ± 5.17	25.48 ± 4.43
Others	ND	0.13

^aData represent the mean of three replications. 

^bCells were grown in 100 ml of TGY or TSB medium at 37°C until ST (A₆₀₀ = 1.0), centrifuged, and washed once with sterile distilled water. Wet cells (40 to 80 mg) were used in the preparation and analysis of FAME as described in Materials and Methods. 

^cn, location of double bond; Δ, location of cyclopropane ring; c, cis. 

^dT_c, phospholipid gel to liquid-crystalline-phase transition temperature (25). 

^eND, not detected (species absent or below detection limit). 

^fData are the total of the mean values ± the standard deviation of replicate values.