TABLE 3.

Effect of growth conditions on D values of Pediococcus sp. at 60°C

Culture growth conditionsa	D value (min)b	Lag time (min)c	pH at end of growthd
TGY, ME, 28°C	0.51 ± 0.04	NDe	6.34
TGY, ST, 28°C	1.09 ± 0.06	ND	5.15
TGY, ST, 37°C	2.81 ± 0.38	0.25	4.86
TSB, ME, 28°C	0.71 ± 0.02	0.25	6.17
TSB, ST, 28°C	4.46 ± 0.06	1.00	5.28
TSB, ST, 37°C	5.28 ± 1.75	2.00	4.68
TGYG, ST, 28°C	0.70 ± 0.02	ND	4.28

^aCells were grown in the appropriate medium (TGY, TSB, or TGYG) until ME or ST at the appropriate temperature (28 or 37°C), centrifuged, washed once with sterile distilled water, and resuspended in tap water to a final cell concentration of ca. 8 log CFU/ml. 

^bData represent the mean values of two replications ± the standard deviation of replicate values. 

^cInitial period for thermal resistance. 

^dMedium pH was measured prior to harvesting of the cells. 

^eND, not detected.