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. 2022 Apr 24;14(5):928. doi: 10.3390/pharmaceutics14050928

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) Cumulative Ce6 release from dHA-Azo-Ce6 and dHA-Ce6 with or without azo-reductase (Na₂S₂O₄) treatment (n = 3, as multiple experiments). (b) The emission spectra (at λ_ex of 400 nm and λ_em of 600–720 nm) of each sample (equivalent Ce6 10 μg/mL) and free Ce6 (10 μg/mL) in PBS (pH 7.4, 150 mM) with or without azo-reductase (Na₂S₂O₄) treatment. (c) 9,10-dimethylanthracene (DMA) fluorescence change (at λ_ex 360 nm and λ_em 380–550 nm) of each sample (equivalent Ce6 10 μg/mL) and free Ce6 (10 μg/mL) in PBS (pH 7.4, 150 mM) with or without azo-reductase (Na₂S₂O₄) treatment.

(a) Cumulative Ce6 release from dHA-Azo-Ce6 and dHA-Ce6 with or without azo-reductase (Na₂S₂O₄) treatment (n = 3, as multiple experiments). (b) The emission spectra (at λ_ex of 400 nm and λ_em of 600–720 nm) of each sample (equivalent Ce6 10 μg/mL) and free Ce6 (10 μg/mL) in PBS (pH 7.4, 150 mM) with or without azo-reductase (Na₂S₂O₄) treatment. (c) 9,10-dimethylanthracene (DMA) fluorescence change (at λ_ex 360 nm and λ_em 380–550 nm) of each sample (equivalent Ce6 10 μg/mL) and free Ce6 (10 μg/mL) in PBS (pH 7.4, 150 mM) with or without azo-reductase (Na₂S₂O₄) treatment.