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. 2022 Apr 22;10(5):881. doi: 10.3390/microorganisms10050881

Figure 2.

Figure 2

Effects of NZX and P2 on the cell membrane: (a) Effects of NZX and P2 on integrity of S. agalactiae ACCC 61733 cells membrane. The membrane penetration rates of NZX and P2 to S. agalactiae cells were detected by flow cytometer under the concentration of 1×, 2×, and 4× MIC for 30 min and 2 h, respectively. (b) Changes in the concentration of extracellular K+ of S. agalactiae ACCC 61733 cells caused by NZX and P2. The concentration of extracellular K+ of S. agalactiae was detected by atomic absorption spectrometer under treatment with 5× MIC NZX and P2 for 0–2 h, respectively. (c) ATP release of S. agalactiae ACCC 61733 cells caused by NZX and P2. ATP content of S. agalactiae cells was measured using a multifunctional microplate reader after treatment with 1–16× MIC NZX and P2 for 1 h, respectively. (d) Changes of membrane potential of S. agalactiae ACCC 61733 cells caused by NZX and P2. Membrane potential of S. agalactiae ACCC 61733 cells was determined by multifunctional microplate reader under the concentration of 0.5–8× MIC NZX and P2 for 30 min. CK was the untreated group.