TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| E. coli | ||
| S17.1 | recA pro thi hsdR RP4-2-Tc::Mu-Km::Tn7 Tra+ Tpr Smr | 50 |
| HB101 | recA13 proA leuB6 hsdR rpsL20 (Smr) | 13 |
| CC118λpir | Δ(ara-leu) araDE ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE (Am) recA1 λpir; phage lysogen | 29 |
| DH5α | supE44 thi-1 recA1 hsdR17 endA1 gyrA (Nalr) Δ(lacIZYA-argF)U169 deoR [φ80 dlacΔ(lacZ)M15] | 27 |
| Pseudomonas sp. strain M1 | Myr+ | This study |
| P. putida NCIMB 8248 | NCIMB wild-type strainb | |
| Pseudomonas sp. strain N22 | Myr− mutant obtained by transposon mutagenesis | This study |
| pRK2013 | RK2-Tra+ RK2-Mob+ Kmrori ColE1 | 22 |
| pUT mini-Tn5 Km | Apr Kmr; ori R6K RP4 oriT delivery plasmid for mini-Tn5 Km | 20 |
| pBluescript KS(+) | Apr α-lac/MCS | Stratagene |
| pLAFR3 | Broad-host-range cosmid vector IncP1 RK2 replicon; λcos+lacZα Mob+ Tra− Tcr | 9 |
| pC7 | Tcr; pLAFR3 with a 22-kb chromosomal fragment from Pseudomonas sp. strain M1 | This study |
| pB22Sc | Apr Kmr; pBluescript KS(+) with the 7.7-kb SacI fragment containing mini-Tn5 | This study |
| pB2.3 BgSc | Apr; pBluescript KS(+) with the 2.3-kb BglII-SacI fragment from pB22Sc | This study |
| pB5.3 Sc | Apr; pBluescript KS(+) with the 5.3-kb SacI fragment from pC7 | This study |
a
Myr, myrcene-degradative phenotype; Apr, ampicillin resistance; Kmr, kanamycin resistance; Smr, streptomycin resistance; Tcr, tetracycline resistance; MCS, multiple cloning site.
b
NCIMB, National Collections of Industrial and Marine Bacteria, Aberdeen, Scotland, United Kingdom.