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. 2022 May 12;10(5):770. doi: 10.3390/vaccines10050770

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© 2022 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CVB3/TD viruses have VPg covalently attached to genomic RNA. Viral RNA was purified from stocks of the CVB3/28, TD8, and TD50 viruses (10⁵ TCID₅₀ units) and analyzed by Western blotting. Blots were probed with an antibody to PV1 VPg (N10). Lane 1 (PV1 Sabin), lane 2, 5 (CVB3/28), lane 3, 6, 8 (CVB3/TD8), lane 4, 7, 9 (CVB3/TD50). Lanes 1–4, RNA was treated with RNase A/T₁; lanes 5–7, RNA was treated with RNase A/T₁ and proteinase K; lanes 8 and 9, RNA was untreated. Adapted with permission from [50] 2005, American Society for Microbiology.