Figure 4.
CVB3/TD viruses have VPg covalently attached to genomic RNA. Viral RNA was purified from stocks of the CVB3/28, TD8, and TD50 viruses (105 TCID50 units) and analyzed by Western blotting. Blots were probed with an antibody to PV1 VPg (N10). Lane 1 (PV1 Sabin), lane 2, 5 (CVB3/28), lane 3, 6, 8 (CVB3/TD8), lane 4, 7, 9 (CVB3/TD50). Lanes 1–4, RNA was treated with RNase A/T1; lanes 5–7, RNA was treated with RNase A/T1 and proteinase K; lanes 8 and 9, RNA was untreated. Adapted with permission from [50] 2005, American Society for Microbiology.