Figure 6.

Compensatory mutations are unlikely in the stem of the CRE-2C. (A) Sequence analysis using NCBI was used to explore the possibility of compensatory mutations in the CRE-2C of CVB3. Potential amino acid variations resulting from compensatory mutations to restore the CRE-2C RNA structure are noted. All variations in the CRE-2C-encoding sequence that would affect the amino acid sequence of the 2C protein are noted. Only 1 amino acid variation identical to those resulting from compensatory mutations was reported to exist in 429 EV-B 2C aa sequences examined (box and arrows). (B) Location of this compensatory mutation in the CRE-2C secondary structure (box). Reprinted with permission [77], 2016, Elsevier.