TABLE 1.
Reference values for parameters used for comparative analysis of the effects of cell preparation protocols on cell surface propertiesa
| Organism | Electrophoretic mobility (μm-cm/Vs) | Viabilityb (%) | Hydrophobicityc (%) | Attachment (103 cells/cm2) to:
|
% Retention on Sepharose columns
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stainless steel | Aluminum | Perspex | Polypropylene | Sepharose | Phenyl-Sepharose | Octyl-Sepharose | DEAE-Sepharose | Carboxymethyl-Sepharose | ||||
| Carbon-limited strain SW8 | −0.38 ± 0.01 | 85.4 ± 3.8 | 92.8 ± 1.9 | 5.2 ± 1.2 | 6.9 ± 2.4 | 2.0 ± 0.7 | 3.1 ± 0.9 | 57.4 ± 5.8 | 60.7 ± 7.7 | 80.6 ± 1.9 | 100 | 19.1 ± 3.3 |
| Nitrogen-limited strain SW8 | −0.51 ± 0.01 | 94.4 ± 3.8 | 92.2 ± 4.6 | 3.1 ± 0.9 | 3.7 ± 1.0 | 1.7 ± 0.6 | 1.2 ± 0.5 | 24.7 ± 1.6 | 41.9 ± 0.7 | 94.7 ± 0.6 | 100 | 22.6 ± 3.1 |
| E. coli | −3.33 ± 0.01 | 89.1 ± 3.7 | 77.6 ± 7.5 | 5.9 ± 2.2 | 7.5 ± 2.0 | 7.9 ± 1.6 | 0.9 ± 0.6 | 42.8 ± 2.3 | 45.3 ± 3.7 | 39.4 ± 1.0 | 91.3 ± 1.8 | 53.7 ± 5.1 |
| S. epidermidis | −1.04 ± 0.01 | 92.4 ± 3.8 | 91.3 ± 1.6 | 4.2 ± 1.2 | 8.2 ± 1.3 | 3.3 ± 1.1 | 0.4 ± 0.2 | 20.0 ± 2.7 | 26.3 ± 1.7 | 36.3 ± 2.1 | 97.7 ± 0.2 | 14.8 ± 2.3 |
a
Cells were centrifuged at 5,000 × g and then washed and resuspended in buffered solutions containing NaCl.
b
Percent viability [(number of CFU on nutrient agar)/(total number of cells)] × 100.
c
Percent hydrophobicity (MATH assay) = [(final optical density of the aqueous phase)/(optical density of the initial suspension prior to mixing with dodecane)] × 100.