TABLE 4.

Impact of cell preparation protocols on the retention on Sepharose columns of test organisms exposed to different combinations of washing and resuspension media

Organism	Treatment	% of reference valuea
		NaCl/NaClb					NaCl/H2O					H2O/NaCl					H2O/H2O
		Sepharose	PhenylSepharose	OctylSepharose	DEAESepharose	CarboxymethylSepharose	Sepharose	PhenylSepharose	OctylSepharose	DEAESepharose	CarboxymethylSepharose	Sepharose	PhenylSepharose	OctylSepharose	DEAESepharose	CarboxymethylSepharose	Sepharose	PhenylSepharose	OctylSepharose	DEAESepharose	CarboxymethylSepharose
Carbon-limited strain SW8	15,000 × gc	100	100	100	100	100	51	60	48	90	215	100	100	88	100	100	100	100	74	100	100
	Freeze-drying	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	136	146	100	100	100	100	100	67	100	100
	Air drying	55	35	65	100	100	25	26	50	100	178	52	63	90	100	100	38	21	17	100	73
Nitrogen-limited strain SW8	15,000 × gc	100	1,714	100	100	100	100	151	275	100	100	100	138	100	100	100	55	100	52	100	100
	Freeze-drying	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	243	161	100	100	178	304	408	352	100	234
	Air drying	167	213	100	100	299	210	215	379	100	165	100	142	100	100	148	203	227	224	100	171
E. coli	15,000 × gc	169	159	208	76	143	100	192	100	100	100	100	100	100	100	211	100	100	100	100	100
	Freeze-drying	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	66	100	100	100	143	364	166	178	100	333
	Air drying	57	100	100	100	100	100	100	20	100	100	100	100	100	100	178	276	134	182	100	189
S. epidermidis	15,000 × gc	209	360	273	100	262	133	100	87	100	100	40	92	83	44	55	401	169	156	100	38
	Freeze-drying	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—e	—e	—e	—e	—e	—e	—e	—e	—e	—e
	Air drying	100	147	100	100	100	21	18	12	100	44	58	100	100	72	47	224	100	100	100	49

^aThe values are percentages of reference values determined after centrifugation at 5,000 × g. 

^bWashing medium/resuspension medium. 

^cCentrifugation at 15,000 × g. 

^dThe bacterial cells used for XPS analysis were not washed in the NaCl solution. 

^eFreeze-dried S. epidermidis cells could not be resuspended.