TABLE 5.

Impact of modification of the resuspension medium on viability, electrophoretic mobility, and hydrophobicity of test organisms subjected to different cell preparation protocols

Organism	Treatment	% of reference value
		Viability		Electrophoretic mobility		Hydrophobicity
		NaCl/H2O (NaCl/NaCl)a	H2O/H2O (H2O/NaCl)	NaCl/H2O (NaCl/NaCl)	H2O/H2O (H2O/NaCl)	NaCl/H2O (NaCl/NaCl)	H2O/H2O (H2O/NaCl)
Carbon-limited strain SW8	5,000 × gb	100	100	271	240	86	74
	15,000 × gc	100	100	246	210	24	69
	Freeze-drying	—d	100	—d	0	—d	17
	Air drying	100	100	281	270	82	100
	MATH	100	100	45	38
Nitrogen-limited strain SW8	5,000 × gb	100	100	490	490	73	88
	15,000 × gc	100	100	460	458	14	75
	Freeze-drying	—d	100	—d	−108e	—d	17
	Air drying	100	100	276	236	88	100
	MATH	100	100	9	32
E. coli	5,000 × gb	100	87	145	564	50	66
	15,000 × gc	100	100	708	375	58	38
	Freeze-drying	—d	100	—d	478	—d	100
	Air drying	100	100	418	521	16	0
	MATH	55	100	505	151
S. epidermidis	5,000 × gb	100	100	435	328	93	100
	15,000 × gc	100	100	1,362	672	100	100
	Freeze-drying	—d	100	—d	—d	—d	—d
	Air drying	100	100	5	88	91	100
	MATH	83	100	9	177

^aWashing medium/resuspension medium (washing medium/resuspension medium with which the reference value was obtained). 

^bCentrifugation at 5,000 × g. 

^cCentrifugation at 15,000 × g. 

^dThe bacterial cells used for XPS analysis were not washed in the NaCl solution. 

^eReversal of the electrophoretic mobility sign resulted in a reduction in the value.