TABLE 6.

Impact of modification of the resuspension medium on interaction with Sepharose columns and attachment to solid substrata of test organisms subjected to different cell preparation protocols

Organism	Treatment	% of reference value
		NaCl/H2O (NaCl/NaCl)a					H2O/H2O (H2O/NaCl)					NaCl/H2O (NaCl/NaCl)				H2O/H2O (H2O/NaCl)
		Sepharose	PhenylSepharose	OctylSepharose	DEAESepharose	CarboxymethylSepharose	Sepharose	PhenylSepharose	OctylSepharose	DEAESepharose	CarboxymethylSepharose	Stainless steel	Aluminum	Perspex	Polypropylene	Stainless Steel	Aluminum	Perspex	Polypropylene
Carbon-limited Psychrobacter sp. strain SW8	5,000 × gb	100	100	109	100	100	100	100	100	100	100	13	13	10	13	100	175	400	383
	15,000 × gc	57	62	57	90	218	100	100	90	100	100	27	13	8	0	42	100	500	200
	Freeze-drying	—d	—d	—d	—d	—d	75	62	67	100	195	—d	—d	—d	—d	100	100	100	100
	Air drying	53	100	100	100	211	100	37	20	100	100	29	100	14	100	100	100	100	0
Nitrogen-limited Psychrobacter sp. strain SW8	5,000 × gb	72	63	26	100	137	57	33	24	100	65	32	46	24	17	100	300	100	100
	15,000 × gc	100	56	69	100	184	35	20	13	100	52	40	38	12	0	52	225	100	0
	Freeze-drying	—d	—d	—d	—d	—d	71	84	85	100	86	—d	—d	—d	—d	30	100	100	22
	Air drying	100	64	95	100	75	100	53	55	100	75	34	56	100	100	11	5	0	0
E. coli	5,000 × gb	56	49	178	110	50	11	26	22	104	31	266	532	35	0	100	2,642	10	100
	15,000 × gc	35	59	73	144	45	15	19	23	109	15	100	33	42	0	540	256	0	0
	Freeze-drying	—d	—d	—d	—d	—d	58	36	34	100	73	—d	—d	—d	—d	25	—e	40	100
	Air drying	63	68	32	100	100	32	34	38	118	33	100	46	100	43	36	44	0	25
S. epidermidis	5,000 × gb	403	317	257	100	666	14	15	18	108	56	100	63	100	0	136	100	100	0
	15,000 × gc	237	82	82	103	256	143	27	34	246	38	212	66	100	0	100	100	39	0
	Freeze-drying	—d	—d	—d	—d	—d	—f	—f	—f	—f	—f	—f	—d	—d	—d	—f	—f	—f	—f
	Air drying	100	39	25	100	269	55	13	12	150	58	43	100	100	100	35	22	0	100

^aWashing medium/resuspension medium (washing medium/resuspension medium with which the reference value was obtained). 

^bCentrifugation at 5,000 × g. 

^cCentrifugation at 15,000 × g. 

^dThe bacterial cells used for XPS analysis were not washed in the NaCl solution. 

^eNo cells adhered to the reference substratum. 

^fFreeze-dried samples of S. epidermidis could not be resuspended.