TABLE 8.

Impact of modification of the washing medium on interaction with Sepharose columns and attachment to solid substrata of test organisms subjected to different cell preparation protocols

Organism	Treatment	% of reference value
		H2O/NaCl (NaCl/NaCl)a					H2O/H2O (NaCl/H2O)					H2O/NaCl (NaCl/NaCl)				H2O/H2O (NaCl/H2O)
		Sepharose	PhenylSepharose	OctylSepharose	DEAESepharose	CarboxymethylSepharose	Sepharose	PhenylSepharose	OctylSepharose	DEAESepharose	CarboxymethylSepharose	Stainless steel	Aluminum	Perspex	Polypropylene	Stainless steel	Aluminum	Perspex	Polypropylene
Carbon-limited Psychrobacter sp. strain SW8	5,000 × gb	100	100	115	100	241	100	100	112	100	203	100	27	15	19	629	778	600	575
	15,000 × gc	100	100	110	100	197	167	140	174	100	100	100	100	8	21	100	600	500	100
	Freeze-drying	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d
	Air drying	100	203	260	100	155	146	100	37	100	83	39	100	43	100	100	100	100	0
Nitrogen-limited Psychrobacter sp. strain SW8	5,000 × gb	136	146	106	100	188	100	100	100	100	100	100	37	100	33	310	282	275	250
	15,000 × gc	154	134	101	100	240	100	47	19	100	68	165	43	59	100	213	257	550	100
	Freeze-drying	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d
	Air drying	79	100	100	100	100	100	80	58	100	100	255	256	400	—e	100	22	0	0
E. coli	5,000 × gb	219	188	221	100	100	42	100	27	100	53	10	9	51	33	7	46	14	100
	15,000 × gc	130	126	110	132	125	58	40	34	100	43	459	233	26	13	2,005	1,911	0	100
	Freeze-drying	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d
	Air drying	362	213	207	100	194	182	100	245	100	78	167	37	150	17	48	35	0	10
S. epidermidis	5,000 × gb	404	326	272	94	588	14	17	9	100	49	100	65	24	100	100	100	46	100
	15,000 × gc	78	94	83	42	123	47	31	34	100	19	100	70	220	100	100	100	100	100
	Freeze-drying	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d	—d
	Air drying	263	249	208	67	256	151	100	100	100	55	66	100	557	19	54	17	0	37

^aWashing medium/resuspension medium (washing medium/resuspension medium with which the reference value was obtained. 

^bCentrifugation at 5,000 × g. 

^cCentrifugation at 15,000 × g. 

^dThe bacterial cells used for XPS analysis were not washed in the NaCl solution. 

^eNo cells adhered to the reference substratum.