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Purification of H5N6 VLPs by sucrose gradient. (A) Purification steps for H5N6 VLPs purification. The H5N6 VLPs were precipitated by a 20% sucrose cushion first and further purified by a 20–60% sucrose density gradient. (B) A visible band at 45% of sucrose fraction indicated the purified H5N6 VLPs.
