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. 2022 May 13;23(10):5473. doi: 10.3390/ijms23105473

Figure 2.

Figure 2

Effects of TGF-β2 on the physical properties, sizes and stiffness of 3D HRPE spheroids under normoxia or hypoxia conditions. Three-dimensional HRPE spheroids at Day 6 prepared under normoxia or hypoxia conditions were treated without (control) or with a 5 ng/mL solution of TGF-β2 (TGF). The mean sizes of 3D HRPE spheroids were measured and each relative ratio against the normoxia conditions without TGF-β2 are plotted in panel (A). The physical solidity of the 3D HRPE spheroids was analyzed by a micro-squeezer and the force required to produce a 50% deformity of a single spheroid during a period of 20 s (μN/μm) was plotted in panel (B). All experiments were performed in triplicate using fresh preparations consisting of 16 spheroids each. “+” is reagents addition. Data are presented as the arithmetic mean ± standard error of the mean (SEM). * p < 0.05 control vs. TGF, § p < 0.05 normoxia vs. hypoxia (ANOVA followed by a Tukey’s multiple comparison test).