Figure 5.

Effects of TGF-β2 on mRNA expression of ECMs of 3D HRPE spheroids under normoxia or hypoxia conditions. Three-dimensional HRPE spheroids at Day 6 prepared under normoxia or hypoxia conditions were treated without (control) and with a 5 ng/mL solution of TGF-β2 (TGFβ), and each sample was subjected to qPCR analysis and the expression of mRNA in ECMs, COL1, COL4, COL6, and FN were estimated. All experiments were performed in duplicate using fresh preparations (n = 10 spheroids each). “+” is reagents addition. Data are presented as the arithmetic mean ± the standard error of the mean (SEM). * p < 0.05 control vs. TGF, § p < 0.05 normoxia vs. hypoxia (ANOVA followed by a Tukey’s multiple comparison test).