TABLE 1.

Purification of α-glucosidase activity from Rhizobium sp. strain USDA 4280

Purification stepa	Vol (ml)	Protein concn (mg/ml)	Total amt of protein (mg)	Activity (μmol/min)b	Sp act (U/mg)	Purification (fold)	Yield (%)
Dialyzed protein extract	50	4.75	238	2.9	0.012		100
DEAE-TrisAcryl M	60	0.346	21	2.4	0.110	9.2	82
Phenyl-Sepharose	5.4	1.180	6.4	0.87	0.73	60.8	30
Reactive Blue 2	5.4	0.202	1.09	0.82	0.75	62.5	28
Ultrogel AcA 44	12.2	0.007	0.091	0.52	5.71	475	18

^aAn 11-g bacterial pellet was used for purification. 

^bα-Glucosidase activity was assayed with 10 mM 4-MUF α-glucoside in PBS at 37°C.