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. 2022 Apr 21;10(5):856. doi: 10.3390/microorganisms10050856

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Root material and analysis scheme to investigate the microbiome and mycobiome of healthy and diseased soybean roots. (A) Soybean roots were collected from healthy plants and plants showing symptoms of taproot decline (TRD) at incipient (S1), moderate (S2), and advanced (S3) stages of taproot decline disease. Representative images of the roots before processing are shown. (B) Root samples and bulk soil collected from the same field site were processed to obtain the microbial and fungal communities of the soil (S), root rhizosphere (R), and endosphere (E). The composition of all samples was investigated using amplicon metagenomics (16S rRNA sequencing and internal transcribed spacer (ITS) regions 1–4 sequencing).