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. 2022 May 21;23(10):5773. doi: 10.3390/ijms23105773

Figure 5.

Figure 5

TEM analysis of cells infected with WT HCMV or ΔUL34 virus. (A) Representative electron micrograph of an MRC5 cell infected with WT AD169-GFP virus. 5 DPI, MOI = 1, scale bar = 10 μm. (B,C) Inset areas from (A) show the cytoplasmic vAC and nucleus, respectively. Scale bars = 2 μm. (D) Representative electron micrograph of an MRC5 cell infected with ΔUL34 AD169-GFP virus. 5 DPI, MOI = 1, scale bar = 10 μm. (E,F) Inset areas from (D) show the cytoplasmic vAC and nucleus, respectively. Scale bars = 2 μm. (G,H) Representative electron micrographs depicting the distinct capsid types in the nuclei of MRC5 cells infected with WT or ΔUL34 AD169-GFP virus, respectively. White arrowhead: A capsids, Black arrowhead: B capsids, Black arrow: C capsids, 5 DPI, MOI = 1, scale bars = 500 nm. (I) Bar chart depicting the total number of each capsid type (A, B or C) in WT infected nuclei cross-sections. Bars = SD, n = 10. (J) Bar chart depicting the total number of each capsid type in ΔUL34 infected nuclei cross-sections. Bars = SD, n = 12. (K–M) Percentage of B, A, and C capsids in WT or ΔUL34 infected nuclei cross-sections shown in (I,J). WT n = 10, ΔUL34 n = 12, bars = SD, * p < 0.05, *** p < 0.001, two-tailed t-test with Welch’s correction.