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. 2022 Apr 24;10(5):677. doi: 10.3390/vaccines10050677

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Age-dependent dynamics of maternally derived antibodies (MDA) in the sera of sows and piglets determined via SP O, A ELISA. To understand the properties of MDAs, five sows and 10 piglets from each sow were screened. For sows, blood was collected 7 days before farrowing. For the piglets born to sows, blood was collected on 7, 14, 28, 42, 56, 84, and 112 days post-birth. Serum antibody titer was determined via SP O, A ELISA using the PrioCheck^TM kit and VDPro^® kit. Data are presented as the mean ± SEM of triplicate measurements (n = 5 for sows and 10 piglets/sow). Data are shown in scatter plots and heat maps. The black dotted line represents the threshold of antibody positive (+) or negative (−). (A–E) represent: (A) Strategy for this study; (B) SP O ELISA determined using the PrioCheck^TM kit; (C) SP O ELISA determined using the VDPro^® kit; (D) SP A ELISA determined using the PrioCheck^TM kit; (E) SP A ELISA determined using the VDPro^® kit.

Age-dependent dynamics of maternally derived antibodies (MDA) in the sera of sows and piglets determined via SP O, A ELISA. To understand the properties of MDAs, five sows and 10 piglets from each sow were screened. For sows, blood was collected 7 days before farrowing. For the piglets born to sows, blood was collected on 7, 14, 28, 42, 56, 84, and 112 days post-birth. Serum antibody titer was determined via SP O, A ELISA using the PrioCheck^TM kit and VDPro^® kit. Data are presented as the mean ± SEM of triplicate measurements (n = 5 for sows and 10 piglets/sow). Data are shown in scatter plots and heat maps. The black dotted line represents the threshold of antibody positive (+) or negative (−). (A–E) represent: (A) Strategy for this study; (B) SP O ELISA determined using the PrioCheck^TM kit; (C) SP O ELISA determined using the VDPro^® kit; (D) SP A ELISA determined using the PrioCheck^TM kit; (E) SP A ELISA determined using the VDPro^® kit.