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. 2022 Apr 24;10(5):677. doi: 10.3390/vaccines10050677

Figure 3.

Figure 3

Time kinetics and half-life of maternally derived antibodies (MDA) as measured via SP O, A ELISA. To understand the properties of MDAs, five sows and 10 piglets from each sow were screened. For sows, blood was collected 7 days before farrowing. For piglets born to sows, blood was collected on 7, 14, 28, 42, 56, 84, and 112 days post-birth. Antibody titer in the sampled serum was measured via SP O, A ELISA using PrioCheckTM and VDPro® kits. Data are presented in regression curves for time kinetics and half-life of MDAs. The red dotted line and the green solid line represent the threshold of antibody positive (+) or negative (−), and the half-life of MDAs, respectively. (A–H) represent: (A,B) Time kinetics and half-life of MDAs determined via SP O ELISA using the PrioCheckTM kit; (C,D) time kinetics and half-life of MDAs determined via SP O ELISA using the VDPro® kit; (E,F) time kinetics and half-life of MDAs determined via SP A ELISA using the PrioCheckTM kit; (G,H) time kinetics and half-life of MDAs determined via SP A ELISA using the VDPro® kit. The data represent the mean ± SEM of triplicate measurements (n = 5 for sows or 10 piglets/sow).