Figure 4.

Pink1 deficiency alleviates collagen antibody-induced arthritis in mice. Three days, 14 days, and 21 days after injection with collagen antibody cocktail, mice received intraperitoneal (i.p) injections of 50 μg lipopolysaccharide. Arthritis incidence and severity were assessed by clinical scoring (a) and by measuring paw thickness (b) from Days 3–28. Disease severity was graded on a scale of 0–4 as described in the materials and methods. Footpad thickness was measured three times per week. (c) Paws were removed from each group of mice after euthanasia, fixed for seven days in 4% paraformaldehyde, decalcified for 21 days in decalcification solution, dehydrated, and embedded in paraffin blocks. Serial sections (10 μm) were cut and stained with hematoxylin and eosin (H&E). Data are expressed as means ± SEM (n = 4 mice/group). * p < 0.05; ** p < 0.01; *** p < 0.001.