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Processing and release of A2d-Bla into the medium. Processing and release of A2d-Bla by B. subtilis DB104(pSB-A2d) were analyzed by pulse-chase labeling at 37°C (A and B) and 25°C (C and D) as described in the legend to Fig. 2. c, cells; s, supernatant; p, pre-A2d-Bla; m, mature A2d-Bla.
