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Improved processing of pre-A2d-Bla by SipS overproduction. Processing and release of A2d-Bla in B. subtilis DB104(pGDL52) (no sipS gene) (A) and B. subtilis DB104(pGDL51) (carries the sipS gene) (B) were analyzed by pulse-chase labeling at 37°C. (Left panels) Samples which include cell-associated and secreted A2d-Bla; (right panels) cellular and medium fractions which were separated by centrifugation after a chase of 2 min. c, cells; s, supernatant; p, pre-A2-Bla; m, mature A2-Bla.
