TABLE 1.
Plasmids and bacterial strains
| Plasmid or strain | Properties or genotype | Source or reference |
|---|---|---|
| Plasmids | ||
| pGA14 | pWVO1 replicon; contains SPO2 promoter; encodes B. licheniformis α-amylase (AmyL) without signal peptide; Emr | 24 |
| pS-A2 | As pGA14; encodes pre-A2-AmyL; Emr | This work |
| pSB-A2 | As pGA14; encodes pre-A2-Bla; Emr | This work |
| pSB-A2d | As pGA14; encodes pre-A2d-Bla; Emr | This work |
| pGDL51 | As pGA14; encodes pre-A2d-Bla; carries the sipS gene of B. subtilis and the kanamycin resistance marker from pKM1; Ems Kmr | This work |
| pGDL52 | As pGDL51, lacks the sipS gene; Emr Kmr | This work |
| pKM1 | pUC7 derivative; Apr Kmr | 14 |
| M13mp10HPA | Phage M13mp10 carrying HPA gene | 42 |
| pSH-A2 | As pGA14; encodes A2-HPA; Emr | This work |
| pSH-A13 | As pGA14; encodes A13-HPA; Emr | This work |
| pUR4129 | Plasmid containing SCA-Lys gene | Unilever Research Laboratories |
| pGA2Lys | As pGA14; encodes A2-SCA-Lys; Emr | This work |
| pGA13Lys | As pGA14; encodes A13-SCA-Lys; Emr | This work |
| pKA13lys | As pGA13Lys; carries additional kanamycin resistance gene; Emr Kmr | This work |
| pGLys | As pGA2Lys; lacks signal sequence A2; Emr | This work |
| Strains | ||
| 8G5 | trpC2 tyr his nic ura rib met ade | 3 |
| DB104 | his nprE aprE | 13 |
| DB430 | his nprE aprE bpf isp1 | 6 |
| WB600 | trpC2 nprE nprB apr epr mpr bpr EmR | 44 |