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. 2022 May 17;14(5):1068. doi: 10.3390/pharmaceutics14051068

Figure 4.

Figure 4

Influence of HA acid and CV on in vivo anticancer properties of M1-EVs loaded with DOX in an orthotopic mouse model of 4T1 pro-metastatic breast cancer. Animals were treated with saline solution (control), high dose of DOX (5 mg/kg), 2 mg/kg of M1-DOX (M1-EVs loaded with 20 µg/mL of DOX), and 2 mg/kg of MM1-DOX (MM1-EVs loaded with 20 µg/mL of DOX). (A) Size comparison of the tumors isolated from animals of the four experimental groups. (B) Tumor volume of treated animals during the 21 days of the experiment. (C) Tumor weight in mg of extracted tumors from animals of the four experimental groups. (D) Immunohistochemical (IHC) detection of NF-κB in tumor sections from treated animals. (E) IHC score of NF-κB for the four experimental groups. (F) Relative mRNA expression of NF-κB, (G) CD8, and (H) FADD in tumor sections from treated animals. Data are means ± SEM (n = 5 animals per condition). Non-significant (ns); **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05.