Specific PCR products (A) and hybridization of the products with digoxigenin-labelled probe CARSOND (B) obtained with pure cultures of 19 P. damselae strains and 13 isolates of related species. Lanes M, 1-kb molecular weight marker; lanes 1 to 13, P. damselae subsp. piscicida DI 21, C.1, R46, DS 11, ATCC 17911, ATCC 29690, 619.1, 10831, IT1, 069E, ATLIT-2, EPOY 8803, and MZS 8001, respectively; lanes 14 to 19, P. damselae subsp. damselae RG 91, RG 153, RM 71, CDC 2227-81, ATCC 33539, and ATCC 35083, respectively; lane 20, V. splendidus; lane 21, V. fischeri; lane 22, V. anguillarum; lane 23, V. pelagius; lane 24, V. harveyi; lane 25, V. proteolyticus; lane 26, V. alginolyticus; lane 27, P. leiognathi; lane 28, P. phosphoreum; lane 29, V. metschnikovii; lane 30, V. nereis; lane 31, V. campbelli.