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. Author manuscript; available in PMC: 2022 May 28.
Published in final edited form as: Eur Respir J. 2021 Dec 23;58(6):2003694. doi: 10.1183/13993003.03694-2020

FIGURE 3.

FIGURE 3

AICAR administration improves respiratory function in Br2-exposed mice. Mice were anaesthetised at 24 h post Br2 exposure (600 ppm for 45 min) and arterial blood was collected from the abdominal aorta. AICAR administration improved blood gas post Br2, including a) arterial CO2 tension (PaCO2), c) arterial O2 saturation (SaO2) and d) arterial O2 tension (PaO2). b) AICAR did not improve blood H+ significantly. e) Br2 did not cause HCO3− changes at 24 h. Data are presented as mean±sem, n=5–9 animals per group. Significance was determined by one-way ANOVA followed by Tukey’s post hoc test. f) Male and female C57BL/6 mice were exposed to Br2 (600 ppm, 45 min) and mortality was assessed over 7 days. AICAR-treated mice showed a significant improvement in survival compared to the vehicle-treated mice (n=8–20 animals per group). Administration of AICAR in the absence of injury in animals and cells produced no significant difference to the air control group. Significant changes in survival were determined by Kaplan–Meier Mantel–Cox log-rank test.