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. Author manuscript; available in PMC: 2022 May 28.
Published in final edited form as: Eur Respir J. 2021 Dec 23;58(6):2003694. doi: 10.1183/13993003.03694-2020

FIGURE 6.

FIGURE 6

AMP-activated protein kinase (AMPK) activation does not protect HO-1−/− mice after exposure to Br2. a) Male and female HO-1−/− mice and their wild-type (WT) littermates were exposed to Br2 (600 ppm for 30 min) and mortality assessed over 10 days. AICAR treatment showed no significant protection in HO-1−/− mice. Significance was determined by Kaplan-Meier Mantel-Cox log-rank test (n=4–11 animals per group). b, c) Representative Western blot of HO-1−/− mice lung tissue showing a significant increase in AMPK and phosphorylated AMPK (pAMPK) post AICAR administration. Fold air indicates the change relative to the air control. d–f) AICAR did not reduce the levels of bronchoalveolar lavage fluid (BALF) protein (d), total cell count (e) or neutrophil count (f) in HO-1−/− mice 24 h post Br2 gas exposure. Data are presented as mean±sem, n=5. Significance was determined by one-way ANOVA followed by Tukey’s post hoc test.