Figure 7.

Effects of S724A mutation of IRE1α upon hepatic gene expression programs related to ER stress and lipid metabolism. Quantitative RT–PCR analysis of the mRNA abundance of the indicated genes in livers of Ern1^S724A/S724A mice and their Ern1^WT/WT littermates following treatment with Tm or vehicle (n = 3–6 per group). A, the UPR genes. B, lipogenesis. C, fatty acid β-oxidation. D, TG secretion. Data are presented as the mean ± SD. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 by two-tailed unpaired Student’s t test. ER, endoplasmic reticulum; IRE1α, inositol-requiring enzyme 1α; TG, triacylglyceride; Tm, tunicamycin; UPR, unfolded protein response.