Figure 9.

S724A mutation of IRE1α has no effect upon apoptosis in ER-stressed livers.A, immunohistochemical staining of CHOP protein in liver sections of Ern1^S724A/S724A mice and their Ern1^WT/WT littermates following Tm treatment. The scale bar represents 100 μm. B, immunoblot analysis of CHOP protein in liver nuclear extracts. Lamin A/C was used as the nuclear protein control. C, immunoblot analysis of caspase-3 in livers of mice following Tm treatment for 24 h. Tubulin was used as the loading control. D, representative images of TUNEL staining of liver sections after 24 h of Tm treatment. The scale bar represents 50 μm. CHOP, CCAAT-enhancer binding protein homologous protein; ER, endoplasmic reticulum; IRE1α, inositol-requiring enzyme 1α; Tm, tunicamycin.