Table 1.
List of monoclonal antibodies used for labeling peripheral blood mononuclear cells (PBMCs) for flow cytometry. Abbreviations: FITC—Fluorescein isothiocyanate, PE—Phycoerythrin, AF647—Alexa Fluor 647, PB—Pacific Blue.
| Antibody | Clone | Isotype | Host Species | Fluorochrome | Catalog Number | Supplier | Dilution |
|---|---|---|---|---|---|---|---|
| CD3: CD8 |
CA17.2A12/ YCATE55.9 |
IgG1 | mouse | FITC:PE | DC047 | BioRad | 1:5 |
| CD4: CD8 |
YKIX302.9/ YCATE55.9 |
IgG2a | rat | FITC:PE | DC048 | BioRad | 1:5 |
| CD4 | YKIX302.9 | IgG2a | rat | AF647 | MCA1038A647 | BioRad | 1:5 |
| CD8 | YCATE55.9 | IgG1 | rat | FITC | MCA1039F | BioRad | 1:5 |
| CD21 | CA2.1D6 | IgG1 | mouse | AF647 | MCA1781A647 | BioRad | 1:5 |
| CD62L | FMC46 | IgG2b | mouse | PB | MCA1076PB | BioRad | 1:5 |
| CD45RA | CA4.1D3 | IgG1 | mouse | - | MA5-16612 | Invitrogen | 1:5 |
| IgG1 | M1-14D12 | IgG1 | rat | PE | 12-4015-82 | Invitrogen | 1:5 |
| IFNγ | CC302 | IgG1 | mouse | AF647 | MCA1783A647 | BioRad | 1:5 |
| IL-17A | eBio17B7 | IgG2a,κ | rat | PE | 12-7177-81 | Invitrogen | 1:20 |