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. 2022 May 19;14(10):2120. doi: 10.3390/nu14102120

Figure 8.

Figure 8

TQ induces apoptosis in TNBC cells. The apoptotic effect of TQ in TNBC cell lines is shown in (A–H) (MDA-MB-231) and (I–P) (MDA-MB-468). TQ was applied to both cell lines for 24 h at concentrations ranging from 0 to 30 µM. An experimental medium with DMSO was used to treat the control cells. A Sony SH800 cell sorter was used to evaluate the percentage of apoptotic cells in TQ-treated samples as well as the control, and an Annexin V-FITC apoptosis kit was utilized to mark treated/control cells. The mean ± S.E.M. of three independent investigations with n = 3 each is shown by each data point in the bar graphs. After TQ treatment, the percentage of apoptotic and necrotic cells increased, while the percentage of viable cells decreased. Using one-way ANOVA and Bonferroni’s multiple comparisons test, the significance of the difference between control and each treatment was assessed. The statistically significant differences were shown by, * p < 0.05, *** p < 0.001, and **** p < 0.0001, ns, nonsignificant.