Figure 2.

The construction⁶⁴Cu-MM-302 and its application in lesion detection in the phase I clinical trial (NCT01304797). (A) Schematic depicting remote loading of ⁶⁴Cu into liposomes using the novel gradient-loadable chelator 4-DEAP-ATSC. Heating liposomes above the lipid bilayer phase transition temperature facilitates transmembrane transport of unprotonated 4-DEAP-ATSC, which becomes protonated within the liposome and remains entrapped. (B) Representative PET and fused PET/CT images of ⁶⁴Cu-MM-302 in lesions at different anatomic locations. Intensity scale bars represent deposition from 0 to 10%ID/kg (derived from SUVmedian). The regions of interest used to measure tumor deposition of ⁶⁴Cu-MM-302 are shown in blue or turquoise outlines. ⁶⁴Cu-MM-302 uptake was detected at above muscle background level in lesions of various anatomic locations that are common for HER2-positive metastatic diseases. Figures are adapted based on Refs. [95,101] with permissions. Copyright 2017 American Association for Cancer Research.