FIG. 5.

CSLM xy images showing binding of the C. ensiformis (A) and T. purpureas (B) lectins to cells of strain Q15 grown at 5°C with diesel fuel as the sole carbon source. C. ensiformis lectin appeared to bind to the exterior surfaces of the diesel fuel microdroplets in the biofilm, creating a bright boundary region. There is also some evidence that there were binding sites within the diesel fuel droplets. The T. purpureas lectin binding was extensive at the cell surface and occurred between cells and within the microdroplets. In addition, there is evidence that an emulsion was formed within the microdroplets and in the vicinity of Q15 cells.