TABLE 1.

Effects of carbon source (glucose-acetate or diesel fuel) and temperature (5 or 24°C) on lectin binding to cells and exopolymer of Rhodococcus sp. strain Q15

Lectin-fluor conjugatea	Glycoconjugate(s)	Growth at 5°C on:		Growth at 24°C on:
		Glucose-acetate	Diesel fuel	Glucose-acetate	Diesel fuel
Arachis hypogaea-TRITC	β-Gal(1->3)-GalNAc	No binding	Binding to cell surface and surfaces of diesel fuel microdroplets	No binding	No binding
Canavalia ensiformis A-FITC	α-Glucose, α-mannose	Weak cell surface binding	Binding to cell surface and surfaces of diesel fuel microdroplets	Strong binding to exopolymer	Strong binding to exopolymer
Tetragonolobus purpureas-TRITC	α-l-Fucose	Weak cell surface binding	Binding to cell surface	Weak cell surface binding	No binding
Triticum vulgaris-TRITC	(GlcNAc)2, NeuNAc	Very weak cell surface binding	Weak binding to cells and exopolymer	Weak binding to cells and exopolymer	Weak cell surface binding
Ulex europeaus-TRITC	α-l-Fucose, (GlcNAc)2	No binding	Binding to cell surface	Strong binding to exopolymer	No binding

^aTRITC, tetramethyl rhodamine isothiocyanate; FITC, fluorescein isothiocyanate.