Table 2.
High-performance liquid chromatography (HPLC) conditions used for the detection of fungicides.
| Fungicide | HPLC Method | Retention Time (min) | Absorption Wavelength (nm) |
|---|---|---|---|
| p-Aminobenzoic acid | 1 | 5.6 | 283 |
| Carbendazim | 2 | 20.1 | 254 |
| Difenoconazole | 2 | 20.3 | 254 |
| Dipicolinic acid | 3 | 6.7 | 270 |
| Flusilazole | 2 | 17.3 | 254 |
| Gentamicin | 4 | 35.7 | 350 5 |
| Kojic acid | 3 | 6.1 | 280 |
| Prochloraz | 2 | 19.2 | 254 |
| Quinolinic acid | 3 | 7.5 | 270 |
| Thiophanate methyl | 2 | 9.2 | 254 |
| Thiram | 2 | 12.1 | 254 |
1 HPLC conditions: 1 mL/min flow (column temperature: 25 °C; injection volume: 10 μL). The mobile phase was 20% to 45% CH3CN in H2O from 0 to 5 min, 45% to 50% CH3CN in H2O from 5 to 19 min, 50% to 60% CH3CN in H2O from 19 to 20 min, 60% to 100% CH3CN in H2O from 20 to 23 min, 100% CH3CN from 23 to 27 min, 100% to 20% CH3CN in H2O from 27 to 28 min, and 20% CH3CN in H2O from 28 to 30 min (H2O contained 0.04% trifluoroacetic acid) [27]. 2 HPLC conditions: 1 mL/min flow (column temperature: 25 °C; injection volume: 5 μL). The mobile phase was 30% to 100% CH3CN in H2O from 0 to 30 min, and 30% CH3CN in H2O from 30 to 35 min [29]. 3 HPLC conditions: constant flow, 0.3 mL/min, 0.03 M H2SO4 aqueous solution (column temperature: 60 °C; injection volume: 10 μL) [19]. 4 HPLC conditions: 1 mL/min flow (column temperature: 25 °C; injection volume: 50 μL). The mobile phase was 0% to 60% CH3CN in H2O from 0 to 60 min, 90% CH3CN in H2O from 60 to 70 min, and 0% CH3CN in H2O from 70 to 75 min [30]. 5 After labeling with Marfey’s reagent [30].