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Screening of the third library of inhibitors. Inhibitory potency of compounds 5′, 5a′, 5d′, and 5d″ as compared to their nucleotide counterparts (5, 5a, and 5d) (each at 100 µM). Compound 4 was used as a positive control. Depending on the solubility of compounds, their stock solution was prepared in water (blue) or DMSO (purple), and hence the control reactions for both solvents are shown. The data represents mean value ± SD from duplicate experiment.
