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. 2022 May 18;23(10):5652. doi: 10.3390/ijms23105652

Figure 4.

Figure 4

The curcuminoid improved the oxidative and nitrosative stress. Oxidative stress was evaluated though the determination of superoxide generation by the DHE dye. (A) Shows representative DHE staining (red) in aortic sections and (C) shows quantification of the DAPI (blue)-normalised red fluorescence. Nitrosative stress was evaluated using an anti-nitrotyrosine antibody. Representative aortic sections of nitrotyrosine staining (green) (B) quantification of the green fluorescence (DAPI normalised) (D). Total and phosphorylated eNOS were assessed by WB; representative WB are shown (E); W: Wistar control; GK: Goto-Kakizaki control; Vh: Goto-Kakizaki submitted to vehicle (DMSO) administration; Curcumin: Goto-Kakizaki submitted to curcumin administration (40 mg/Kg/day, s.c.) and Curcuminoid: Goto-Kakizaki submitted to curcuminoid (52.4 mg/Kg/day, s.c.) administration. Results are expressed as mean ± S.E.M.; vertical bars represent S.E.M.; n = 5–9/group; statistical differences were evaluated by Tukey’s test. * vs. W; & vs. GK; # vs. Vh; $ vs. Curcumin. 1 symbol p < 0.05; 2 symbol p < 0.01; 3 symbol p < 0.001.