Table 1.

Primer sets used in conventional PCR for the detection of PRRSV and SIV.

Primer Set	Target Region	Primer Sequence (5′-3′)	Amplicon Length (bp)	Reference
PRRS_Set_1	ORF1b	Forward: CCTCCTGTATGAACTTGC Reverse: AGGTCCTCGAACTTGAGCTG	Type 1 & Type 2 255 bp	[37]
PRRS_Set_2	ORF7	Forward: CCAGCCAGTCAATCARCTGTG Reverse: GCGAATCAGGCGCACWGTATG	Type 1 & Type 2 300 bp	[38]
PRRS_Set_3	ORF7	Forward: ATGGCCAGCCAGTCAATCA Reverse: TCGCCCTAATTGAATAGGTGA	Type 1 398 bp Type 2 433 bp (Discriminative primer set)	[39]
SIV_Set_1	M gene (pan-influenza A)	Forward: GACCRATCCTGTCACCTCTGAC Reverse: AGGGCATTYTGGACAAAKCGTCTA	106 bp	[40]
SIV_Set_2	NP gene (swine influenza)	Forward: GCACGGTCAGCACTTATYCTRAG Reverse: GTGRGCTGGGTTTTCATTTGGTC	200 bp	[41]
SIV_Set_3	H1 swine type hemagglutinin	Forward: GTGCTATAAACACCAGCCTYCCA Reverse: CGGGATATTCCTTAATCCTGTRGC	116 bp	[41]
SIV_Set_4	H3 swine type hemagglutinin	Forward: CTTGATGGRGMAAAYTGCACA Reverse: GGCACATCATAWGGGTAACA	133 bp	[42]
SIV_Set_5	H1 avian type hemagglutinin	Forward: GAAGGRGGATGGACAGGAATGA Reverse: CAATTAHTGARTTCACTTTGTTGC	139 bp	[42]
SIV_Set_6	H1 pandemic hemagglutinin (Pandemic 2009)	Forward: GGGCATTCACCATCCATCTACT Reverse: CCTCACTTTGGGTCTTATTGCTATTT	133 bp	[42]
SIV_Set_7	N1 swine type neuraminidase	Forward: AGRCCTTGYTTCTGGGTTGA Reverse: ACCGTCTGGCCAAGACCA	126 bp	[42]
SIV_Set_8	N2 swine type neuraminidase	Forward: AGTCTGGTGGACYTCAAAYAG Reverse: TTGCGAAAGCTTATATAGVCATGA	116 bp	[42]
SIV_Set_9	N1 pandemic hemagglutinin (Pandemic 2009)	Forward: GGGACAGACAATAACTTCTCAATAAAGC Reverse: TTCAGCATCCAGAACTAACAGGGT	100 bp	[42]