Table 3.
Radiometric kinase assay * of the isolated compounds from Tylophora indica.
| Serial | Compounds | IC50 Values (µM) a | |
|---|---|---|---|
| Aurora-A | Aurora-B | ||
| 1. | Tylophorine | 21.4 ± 1.24 | 18.3 ± 1.32 |
| 2. | Tylophorinicine | 16.2 ± 1.43 | 10.2 ± 1.21 |
| 3. | Tylophorinine | 4.1 ± 1.08 | 3.6 ± 0.93 |
| 4. | Tylophorinine N-Oxide | >100 | >100 |
| 5. | Tylophorinidine | 0.6 ± 0.72 | 1.3 ± 0.58 |
| 6. | Tylophorinidine-N-Oxide | 2.6 ± 0.86 | 6.5 ± 0.76 |
| 7. | Septicine | >100 | >100 |
| 8. | Chlorogenic acid | ND | ND |
| 9. | Chlorogenic acid methyl ester | ND | ND |
a Values are the average of three independent experiments; IC50: Inhibitory concentration for 50%; ND: not determined. * Purified protein kinases were used as positive controls for the assay (Sigma-Aldrich, St. Louis, MO, USA).