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. 2022 May 6;9(5):224. doi: 10.3390/vetsci9050224

Figure 1.

Figure 1

(A) Western blot analysis of ProAKAP4 and AKAP4 expression in post-thawed isolated spermatozoa (SPZ) in post-thawed cryopreservative without spermatozoa (SP). ProAKAP4 and the prodomain (indicated by arrows) were detected in the spermatozoa (SPZ) lane at 100 and 18 kDa but not in the extender (SP) diluted with the cryopreservative (SP) with the 6F12 mouse monoclonal purified antibody. (B) Both proAKAP4 and AKAP4 were detected at 100 and 82 kDa with the 7E10 mouse monoclonal purified antibody. No labelling was observed in the lane with extender with cryopreservative without spermatozoa (SP lanes). Apparent molecular weights were indicated on the left of the SDS-PAGE lanes and determined using calibrated molecular weight markers. The whole Western blot figure in the Supplementary Figure S1.