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. 2022 May 10;14(5):334. doi: 10.3390/toxins14050334

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Multiple sequence alignments of cytotoxins identified (NK-CTX, NS-CTX, and NA-CTX), and the sequences of cytotoxin matched in database. Matched amino acid sequences are in red. Dashed lines represent amino acids that were not covered by nano-ESI-LCMS/MS. Arrow in blue depicts Serine28 of S-type cytotoxin, and arrow in red depicts Proline30 of P-type cytotoxin. UniProt ID: P01446 is cytotoxin 3 from N. kaouthia, A0A7T7DMY7 is cytotoxin 1 from N. sumatrana, P60301 is cytotoxin 3 from N. atra.