Figure 6.

Cytotoxicity of purified cytotoxins from N. kaouthia (NK-CTX), N. sumatrana (NS-CTX), and N. atra (NA-CTX) venoms and neutralization with NkMAV, SAV, and NBAV on CRL-2648 cell line. Cell viability plot of NK-CTX (A), NS-CTX (B), and NA-CTX (C) in mouse fibroblast cell lines and neutralization with NkMAV, SAV, and NBAV. Average IC₅₀ of cobra cytotoxins, NK-CTX, NS-CTX, and NA-CTX and neutralization with antivenom (D). The assay represents biological and technical triplicates. One-way ANOVA with Tukey’s post hoc test was used in determining statistical significance. ** and *** represents statistical significance between venom IC₅₀ and antivenom neutralized IC₅₀ within the respective cobra group, p < 0.001 and p < 0.0001 respectively. ## represents statistical significance between venom IC₅₀ of cobra species, p < 0.001.