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. 2022 May 4;15(5):574. doi: 10.3390/ph15050574

Figure 2.

Figure 2

Breast cancer cells induce myeloid NLRP3 and PD-L1. (A,B) THP-1 or THP-1-NLRP3def cells were stimulated with MDA-MB-468-conditioned media (+BCM). (A) Mean ± SEM IL-1β production from THP-1 or THP-1-NLRP3def cells left unstimulated (RPMI) or treated with conditioned media after 48 h (n = 3). (B) Mean ± SEM of relative gene expression of Pdcd1l1 from cells described in (A,B) or stimulated with (+BCM) and treated with OLT1177 (n = 3). (C–G) Bone marrow adherent cells stimulated with 4T1-conditioned media (+BCM). (C) Representative Western blot images from (C–G); mean ± SEM of PD-L1/β-actin ratio (D), NLRP3/β-actin ratio (E), pro-IL-1β/β-actin ratio (F) and mature IL-1β/β-actin ratio (G) from cells described in (C–G) (n = 3). *** p < 0.001, ** p < 0.01.