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. 2022 May 19;10(5):803. doi: 10.3390/vaccines10050803

Figure 2.

Figure 2

Spike protein production by engineered L. tarentolae. The presence of the spike protein on the parasite was evaluated by immunofluorescence staining using a SARS-CoV-2 spike antibody and the Alexa Fluor 488-conjugated anti-rabbit IgG secondary antibody. Green dots show the presence of the spike protein, while membranes are labeled in red using Concanavalin A staining. Yellow/orange dots are the result of the overlapping of the two signals (orthogonal projection) and are indicative of the colocalization of the spike protein on the membrane. The nucleus and kinetoplastid DNA was stained with DAPI (blue).