Figure 2.

Infectious particles are excreted in murine breast milk. (A): Female dams were MOCK or ZIKV-infected with the Brazil/2016 strain as described in Figure 1. Breast milk samples were collected at 4 days post-infection and centrifuged to separate the cream fraction, the whey, and the cell pellet. The cream and whey fractions were isolated and further centrifuged twice with PBS to increase their purity. (B): The concentration of infectious viral particles in whole breast milk or breast milk fractions was determined by plaque assays (whole milk: n = 11; cream and whey: n = 5).