Figure 6.
Free fatty acids are antiviral against ZIKV. (A): The fatty acids tested are either saturated fatty acids (SFA), mono-unsaturated fatty acids (MUFA), or polyunsaturated fatty acids (PUFA). Their nomenclature, structure, and abundance in human milk at 4 weeks after delivery (Cruz-Hernandez, 2013) are listed in the table. (B): To determine whether free fatty acids exert antiviral activity, 106 PFU of ZIKV particles (Brazil/2016) were incubated with culture medium (NT), paraformaldehyde 4% (PFA), free fatty acids (FFA), or the solvent used to dilute them (solvent) for 6 h. At several time points (0 h, 1 h, 4 h, and 6 h), the viral titers were determined by plaque assays (n = 3).