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(A) For staining the GSCs, pMad antibody was used. (B) For staining single dots containing spectrosomes and fusomes, we used α-Spectrin. (C) CBs were differentiated from the GSCs and cyst based on the presence of single spectrosome-containing dots. Only GSCs and CBs have single dots containing spectrosomes. To stain DNA, Hoechst was used. Scale bar, 10 μm.
