Figure 3.

Effect of cyromazine on the germ cells of female adult ovary. D. melanogaster was continuously selected with cyromazine from the mid-3rd instar larvae until 3-day-old adults. Later, the number of germ cells were counted from 100 germaria per biological replicate (N = 300). (A) Results showed a 27% decrease in the number of GSCs compared to the control group. (B) A 23% decrease in the number of CBs was observed. (C,D) The germarium of both control and treated groups, respectively, were stained with pMad, α-Spectrin, and Hoechst. Further, the GSCs are indicated by white circles and the CBs are indicated by white arrows. Scale bar, 10 μm. The p-value of Student’s t-test is *** p < 0.001.