FIG. 2.

DGGE band patterns of 16S rDNA PCR amplification products obtained with the CTO_PCR primers (10) and community DNA. DGGE gels were stained with ethidium bromide and photographed under UV illumination. Lanes: 1, 3, 5, and 7, PCR products from 1994 samples; 2, 4, 6, and 8, PCR products from 1995 samples; 1 and 2, tilled, replicate plot 5; 3 and 4, tilled, replicate plot 6; 5 and 6, native (never-tilled), replicate plot 3; 7 and 8, native (never-tilled), replicate plot 4; 9 through 12, PCR products from 1995 samples; 9, fertilized successional microplot within replicate plot 1; 10, unfertilized successional, replicate plot 1; 11, fertilized successional microplot within replicate plot 3; 12, unfertilized successional, replicate plot 3. The arrows on the right indicate the locations of unique bands in lane 12.